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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
Anti Human Igg, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
Secondary Antibodies Goat Anti Human Irdye800cw, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
Anti Human Igg Fc Goat Antibody, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
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biotinylated goat anti human igg antibody  (Vector Laboratories)
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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
Biotinylated Goat Anti Human Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated <t>with</t> <t>anti-human</t> IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.
Biotinylated Goat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated with anti-human IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.

Journal: bioRxiv

Article Title: Proteomic Screening Identifies HNRNPA2B1 as an Epigenetic Repressor of Epstein-Barr Virus Reactivation

doi: 10.64898/2026.02.27.708571

Figure Lengend Snippet: ( A ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata (EBV+) cells transduced with non-targeting control (sg-NC) or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; **p<0.01. ( B ) Akata (EBV+) cells carrying control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1) were treated with anti-human IgG for the indicated times. Protein levels of HNRNPA2B1 and KDM1A were analyzed by WB. β-actin serves as a loading control. ( C ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 cells expressing control sg-NC or HNRNPA2B1-targeting sgRNA (sg2-A2B1). Anti-KDM1A was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. ***p<0.001. ( D ) ChIP-qPCR analysis of KDM1A occupancy at ZTAp and RTAp in Akata-BX1 sgA2B1 cells reconstituted with vector control or HNRNPA2B1 (pLenti-A2B1). Anti-KDM1A antibody was used for KDM1A ChIP and IgG served as a negative control. Data are normalized to 2% input and shown as mean ± SD from three biological replicates. *p<0.05; ***p<0.001.

Article Snippet: After 3 h, cells were lytically induced by anti-human IgG (50 μg/mL; Cat. #0855087, MP Biomedicals) and harvested at the indicated time points as described previously [ , , ].

Techniques: ChIP-qPCR, Transduction, Control, Negative Control, Expressing, Plasmid Preparation